Questão de Biologia e Ciências

Utilize o texto apresentado a seguir para responder à questão: The NucleoSpin® Microbial DNA kit is designed for efficient isolation of genomic DNA from microbial samples. DNA can be isolated from a wide variety of microorganisms such as gram-negative, and gram-positive bacteria as well as yeast, e.g., Escherichia coli, Bacillus subtilis, Corynebacterium glutamicum, Saccharomyces cerevisiae. Preparation of the collected samples containing the microbes of interest should be in pellet format. Preliminary data also indicate the usability of the kit for DNA isolation from fungal mycelia, e.g., Aspergillus nidulans, from bacterial spore suspensions, e.g., Geobacillus stearothermophilus, and from plant pollen, e.g., honey bee pollen baskets. For optimal DNA yield, bead tubes different from the ones included in the kit might be required for such applications (see section 2.4). Microbial samples such as gram-positive bacteria, yeast, and spores can be difficult to lyse due to their strong complex cell wall structures. The NucleoSpin® Microbial DNA kit replaces enzymatic lysis by utilizing mechanical disruption of cell wall structures with the NucleoSpin® Bead Tubes. The NucleoSpin® Bead Tubes can be used in combination with many compatible disruptive devices (see section 2.4.1). High DNA yields can be obtained with the NucleoSpin® Bead Tubes from a large variety of sample types – enabling the procedure to be convenient, fast, and easy. Alternative bead types can be ordered separately for select sample types (see section 2.4.2 for recommendations). Macherey- Nagel. Genomic DNA from microoganisms, user manual NucleoSpin® Microbial DNA, p. 06, 2016. Fonte: <http://www.mn-net.com/Portals/8/attachments/Redakteure_Bio/Protocols/Genomic%20DNA/ UM_gDNAMicrobi>. Acesso em: 14/3/2018. De acordo com o manual de isolamento do DNA genômico para amostras de bactérias Gram-positivas, leveduras e esporos: